Quantitative solid-phase assay to measure deoxynucleoside triphosphate pools
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چکیده
منابع مشابه
Origins of mitochondrial thymidine triphosphate: dynamic relations to cytosolic pools.
Nuclear and mitochondrial (mt) DNA replication occur within two physically separated compartments and on different time scales. Both require a balanced supply of dNTPs. During S phase, dNTPs for nuclear DNA are synthesized de novo from ribonucleotides and by salvage of thymidine in the cytosol. Mitochondria contain specific kinases for salvage of deoxyribonucleosides that may provide a compartm...
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The size of the deoxyribonucleoside triphosphate pools of vitamin B-12-deficient cells of Euglena gracilis, and of vitamin B-12-deficient cells repleted with the vitamin, were measured. We found that the pools were very small, if they exist at all, in deficient cells but expand rapidly with the addition of the vitamin. The sizes of the pools decrease when DNA synthesis is completed, and are ver...
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ژورنال
عنوان ژورنال: Biology Methods and Protocols
سال: 2018
ISSN: 2396-8923
DOI: 10.1093/biomethods/bpy011